Regulatory
spaCBA pro

Part:BBa_K2760005:Design

Designed by: Fernanda Diaz   Group: iGEM18_TecMonterrey_GDL   (2018-10-09)


Promoter spaCBA for Lactobacillus rhamnosus GG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 38
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed and codon optimized for L. rhamnosus GG.


Source

This sequence was taken from the spaCBA pilus operon promoter region. From the coding region of the spaC pilus gene in the L. rhamnosus GG [1].

References

References: [1] Rintahaka, Johanna & Yu, Xia & Kant, Ravi & Palva, Airi & von Ossowski, Ingemar. (2014). Phenotypical Analysis of the Lactobacillus rhamnosus GG Fimbrial spaFED Operon: Surface Expression and Functional Characterization of Recombinant SpaFED Pili in Lactococcus lactis. PloS one. 9. e113922. 10.1371/journal.pone.0113922.